Protocols in molecular biology and epigenetics © Christoph Grunau

• preparation of genomic DNA from tissue samples
• preparation of high molecular weight genomic DNA
• preparation of dNMPs from ds DNA
• preparation of dNMPs from ss DNA
• plasmid preparation using diatomaceous earth
• Prism-Sequencing (PDF)
• Troubleshooting with bad quality sequencing gels (PDF)
• Fluorescence in-situ hybridization (FISH)
• making HT1080 metaphases
• colony PCR
• purification of PCR products (gel filtration)
• purification of PCR products (PEG precipitation)
• southern hybridization with universal alpha-satellite oligonucleotide probe
• SB buffer for fast DNA agarose gel electrophoresis
• High efficiency PCR (after J.Buard & A.Jeffreys)
• RT-PCR troubleshooting for better results in reverse transcription PCR
• SDS-PAGE (link) - and the role of glycine in protein electrophoresis
  (comment: Better use water saturated n-butanol to overlay after pouring the running gel. Butanol is the upper phase, it has a density of about 0.8.)
• Role of Lithium in RNA preparation (link)
• Western Blot of histones
• RT-PCR of individual larvae of Schistosoma mansoni
• Generation of a phage Lambda library of genomic DNA of Schistosoma mansoni