Phosphate-buffered saline (PBS), Dulbeccos formula (pH 7.3)
Solution A (PBSA)
Sodium chloride |
8.00g |
Potassium chloride |
0.20 g |
Di-Sodium hydrogen orthophosphate |
1.15 g |
Potassium di-hydrogen orthophosphate |
0.20 g |
Dissolve in pure water and make up to 800 ml.
Solution B
Magnesium chloride hexahydrate |
0.10 g |
Dissolve in pure water and make up to 100 ml.
Solution C
Calcium chloride dihydrate - 0.10 g
Dissolve in pure water and make up to 100 ml.
Autoclave each solution separately. When cooled, pour solution B into solution A stirring slowly and then add solution C, i.e. eight parts A, one part B, one part C.
Complete Dulbecco's solution is rarely used. For most purposes, the calcium- and magnesium-free solution A (PBSA) suffices.