Phosphate-buffered saline (PBS), Dulbeccos formula (pH 7.3)

Solution A (PBSA)

Sodium chloride	8.00g
Potassium chloride	0.20 g
Di-Sodium hydrogen orthophosphate	1.15 g
Potassium di-hydrogen orthophosphate	0.20 g

Dissolve in pure water and make up to 800 ml.

Solution B

Magnesium chloride hexahydrate

0.10 g

Dissolve in pure water and make up to 100 ml.

Solution C

Calcium chloride dihydrate - 0.10 g
Dissolve in pure water and make up to 100 ml.
Autoclave each solution separately. When cooled, pour solution B into solution A stirring slowly and then add solution C, i.e. eight parts A, one part B, one part C.

Complete Dulbecco's solution is rarely used. For most purposes, the calcium- and magnesium-free solution A (PBSA) suffices.