Sodium boric acid electrophoresis buffer
| 20x SB buffer: | |
| 8g | NaOH |
| 45g | Boric Acid |
| to 1L | with MilliQ water |
In this buffer, agarose gels can be run at higher voltage than in TBE/TAE (typically 10 V/cm). DNA fragment separation is therefore faster.
Ref:
Brody JR, Kern SE. "Sodium boric acid: a Tris-free, cooler conductive medium for DNA electrophoresis." Biotechniques. 2004 Feb;36(2):214-6. [PubMed]